【佳学基因靶向药物基因检测】miR-20a 在具有 PKD1 突变的家猫血清中上调

基因肿瘤检测公司排名解码

探索癌症的早期发现及检测看到《PLoS One》在 2022 Dec 20;17(12):e0279337.发表了一篇题目为《》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Marcela Correa Scalon, Christine Souza Martins, Gabriel Ginani Ferreira, Franciele Schlemmer, Ricardo Titze de Almeida, Giane Regina Paludo等完成。促进了肿瘤的精准治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤基因检测及靶向药物治疗研究关键词：

多囊肾病,PKD,常染色体,显性遗传,多囊肾病,ADPKD,肾实质囊肿,异质性疾病

肿瘤治疗检测基因临床应用结果

多囊肾病 (PKD)，也称为常染色体显性遗传性多囊肾病 (ADPKD)，是一种以肾实质囊肿为特征的遗传异质性疾病。它是家猫最普遍的遗传性疾病。微小 RNA（miRNA 或 ncRNA）是短的非编码单链 RNA，可通过影响许多靶基因以及直接调节 PKD 基因表达来诱导 PKD 细胞发生。我们比较了 9 只 PKD1 突变家猫血浆和血清样本中 miR-20a、-192、-365、-15b-5p 和 -16-5p 的相对表达谱，通过聚合酶链反应 (PCR) 检测，以及对照组 (n = 10)。来自具有 PKD1 突变的猫的血液样本提供了相似浓度的来自血浆或血清的 microRNA。 PKD 组血清 miR-20a 上调，p < 0.005； Roc 曲线分析显示 AUC 为 90.1%，临界值灵敏度为 77.8%，特异性为 100%。该数据提供了有关外周血采样中肾脏 miRNA 表达的重要信息。

肿瘤发生与革命国际数据库描述：

Polycystic kidney disease (PKD), also known as autosomal dominant polycystic kidney disease (ADPKD) is a genetically heterogeneous condition characterized by cysts in renal parenchyma. It is the most prevalent inherited disease of domestic cats. MicroRNAs (miRNAs or ncRNA) are short, noncoding, single-stranded RNAs that may induce PKD cytogenesis by affecting numerous targets genes as well as by directly regulating PKD gene expression. We compared the relative expression profile of miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of nine domestic cats with PKD1 mutation, detected by polymerase chain reaction (PCR), and a control group (n = 10). Blood samples from cats with PKD1 mutation provide similar concentrations of microRNAs either from plasma or serum. Serum miR-20a is upregulated in PKD group with p < 0.005; Roc curve analysis showed an AUC of 90,1% with a cut-off value sensitivity of 77.8% and specificity of 100%. This data provides important information regarding renal miRNA expression in peripheral blood sampling.